![]() ![]() The secondary structure of a nucleic acid molecule refers to the base pairing interactions within one molecule or set of interacting molecules. Structural biologists solving a new atomic-resolution structure will sometimes assign its secondary structure by eye and record their assignments in the corresponding Protein Data Bank (PDB) file. Many other less formal definitions have been proposed, often applying concepts from the differential geometry of curves, such as curvature and torsion. For example, helices can adopt backbone dihedral angles in some regions of the Ramachandran plot thus, a segment of residues with such dihedral angles is often called a helix, regardless of whether it has the correct hydrogen bonds. The secondary structure of a nucleic acid is defined by the hydrogen bonding between the nitrogenous bases.įor proteins, however, the hydrogen bonding is correlated with other structural features, which has given rise to less formal definitions of secondary structure. In proteins, the secondary structure is defined by patterns of hydrogen bonds between backbone amine and carboxyl groups (sidechain–mainchain and sidechain–sidechain hydrogen bonds are irrelevant), where the DSSP definition of a hydrogen bond is used. Secondary structure is formally defined by the hydrogen bonds of the biopolymer, as observed in an atomic-resolution structure. These determine the general three-dimensional form of local segments of the biopolymers, but does not describe the global structure of specific atomic positions in three-dimensional space, which are considered to be tertiary structure. The secondary structure of a protein is the pattern of hydrogen bonds in a biopolymer. Secondary (inset) and tertiary structure of tRNA demonstrating coaxial stacking PDB: 6TNA) Of snoRNAs, LSm binding site found in spliceosomal RNAs such as U1, U2, U4, U5, U6, U12 and U3, the Shine-Dalgarno sequence, Īnd the RNA polymerase III terminator. Some examples of such motifs are: the C/D Often, the primary structure encodes sequence motifs that are of functional importance. The nucleic acid sequence refers to the exact sequence of nucleotides that comprise the whole molecule. The primary structure of a protein is reported starting from the amino N-terminus to the carboxyl C-terminus, while the primary structure of DNA or RNA molecule is known as the nucleic acid sequence reported from the 5' end to the 3' end. For a typical unbranched, un-crosslinked biopolymer (such as a molecule of a typical intracellular protein, or of DNA or RNA), the primary structure is equivalent to specifying the sequence of its monomeric subunits, such as amino acids or nucleotides. The primary structure of a biopolymer is the exact specification of its atomic composition and the chemical bonds connecting those atoms (including stereochemistry). Main articles: Protein primary structure and Nucleic acid sequence The terms primary, secondary, tertiary, and quaternary structure were introduced by Kaj Ulrik Linderstrøm-Lang in his 1951 Lane Medical Lectures at Stanford University. This leads to several recognizable domains of protein structure and nucleic acid structure, including such secondary-structure features as alpha helixes and beta sheets for proteins, and hairpin loops, bulges, and internal loops for nucleic acids. The scaffold for this multiscale organization of the molecule arises at the secondary level, where the fundamental structural elements are the molecule's various hydrogen bonds. This useful distinction among scales is often expressed as a decomposition of molecular structure into four levels: primary, secondary, tertiary, and quaternary. The structure of these molecules may be considered at any of several length scales ranging from the level of individual atoms to the relationships among entire protein subunits. ( PDB: ADNA, 1BNA, 4OCB, 4R4V, 1YMO, 1EQZ)īiomolecular structure is the intricate folded, three-dimensional shape that is formed by a molecule of protein, DNA, or RNA, and that is important to its function. Interactive image of nucleic acid structure (primary, secondary, tertiary, and quaternary) using DNA helices and examples from the VS ribozyme and telomerase and nucleosome. ![]()
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![]() ![]() Yes, running a dehumidifier will add heat energy to your home (just like anything, including running your computer, adds heat energy). At that heat-index reading, you’re at risk of heat-related injury even if you’re sitting still and not exerting yourself. But if it’s 92 ☏ and around 95% humidity, it feels like 127 ☏. ![]() That’s still pretty warm, but it’s not deadly hot, especially if you take it easy. If it’s 92 ☏ and around 40% humidity, it feels like it’s about 94 ☏. The old “It’s not the heat, it’s the humidity!” chestnut people like to trot out is well grounded in science. If you’ve ever heard weather forecasters talking about the heat index or seen the RealFeel temperature on your favorite weather app, you’re seeing the effect of the ambient temperature and the humidity presented in a way that translates to human comfort (and risk). ![]() The higher the humidity, the harder it is to sweat effectively, and the more dangerous high temperatures become. The bulk of them, after all, are purchased to keep damp crawl spaces and basements dry.īut evaporative cooling is the fundamental way humans cool off. Don’t Overlook Dehumidifiers in Damp Climatesĭehumidifiers might not be on your mind when you’re talking about staying cool. While practically every model comes with a simple spacer for a traditional single or double-hung window that can usually be rotated vertically for a slider window, you may need to order an adapter for a taller “window” like a sliding glass door or an awkwardly shaped one like a casement window. By all means, if you’re suffering through a heatwave and only have access to a single-hose model, use it to stay cool, but when buying new look for a dual-hose model. ![]() ![]() ![]() “There’s stuff we’ll never know,” he says. The spiritual conviction was there long before Xavier discovered the unsettled legacy of his paternal great grandmother, who would disappear from public records after being taken from her Wiradjuri homelands to Melbourne. “I don’t need to understand who that is or what that is, I just need to be a clean, strong vessel for that to come through, and be humble about it to realise it’s something that resonates within me and I just need to hold space for it, not let my ego get in the way.” “I’ve always felt like I’ve had this old woman spirit with me that that is prevalent in my music, and I’m a vessel for that old woman to speak,” he explains, not for the first time as others try to understand his innate connection to his craft. And I guess I always celebrate those things in my music.”Īs a child, at one with the sea and sand, inventing songs for his own enjoyment, Xavier set the tone for a life where creation was its own reward, and a gift not taken lightly. I think the roots of who we are, and what our ancestral lines are and how those stories have shaped us are the most important things. I don’t care too much for the other stuff. “I value community, culture, our connection to the earth as human beings. “I value the simple things in life,” he shrugs. Countless more celebrated acts have come and gone like fireworks as his campfire steadily grows: a beacon to the kind of music fan that seeks sustenance in a fast-food world. Today, Xavier’s relentless upward arc is among the most astounding success stories of our time. Those instinctive beginnings eventually yielded the more crafted and purposeful likes of Spirit Bird, Nanna (with his nine-piece band the United Nations), Storm Boy and seven more live albums - though nobody could ever count those early bootlegs swapped by fans from Argentina to the Czech Republic, serving to double his audience on each return. I was experimenting with didges and gaffa-taping them to chairs and I always had this real love for wood and the tones of wood, so it was all very earthy.” “When I first got successful I was surprised, because I thought what I was doing was more like art,” he says. From his birthplace on the blustery southeast coast of Australia to Europe, Japan, the US, Canada and South America, you can measure it today in audiences in their tens of thousands, and in hundreds of millions of song streams, not least his timeless, windswept keynote, Follow The Sun (2012).Īcoustic guitar in hand, engulfed by his progressively more complex and elaborate scaffold of didgeridoos and percussion, Xavier Rudd cut an utterly unique and compelling figure as the world warmed to his first indie albums of the early 2000s, Live In Canada, To Let and Solace. The break accelerated a return to the solo mode of creation that first led the barefoot multi-instrumentalist on his phenomenal journey. I’ve been able to experiment with some new ideas and new sounds…” “I had a chance to reset and change a few things around, to reassess where I was at, musically and practically. “I’d planned to take a year off anyway, the first time in 20 years I hadn’t done an overseas circuit,” he says. The wide-open space he’d found himself in - the great COVID silence - was both beyond his control and curiously in-sync. It felt like a wind of change, literally, in so many ways.” “We were on a trip north to the Cape and the wind was blowing too hard to take the tinnie out to the island… As I contemplated everything in my life and what was happening in the world there was literally a strong southeaster blowing, all the time. “It hit me last year when I started to write Stoney Creek,” the Australian roots journeyman says of the album’s first, exhilarating single: a rolling acoustic balm of a song that finds refuge in the simple blessings of rest, companionship and belonging in a world gone crazy. It’s a recurring image that speaks of wide-open space and the awesome natural elements that shape it: a force far greater than us, but ours to harness if we take the time to learn, reflect and respect its ways. The wind blows strong though Xavier Rudd’s tenth album. ![]() ![]() Once you master independent timed attacks you will automatically master timed attacks where more players are involved and be able give a hand your mate when needed. When doing independent timed attack you depend only on your own skills, and when you make mistake you’ll know where it is and make it right next time. Those attacks is way harder to accomplish because attack depends of several people and there are bigger odds that someone will make mistake. ![]() I want to mention timed attacks where more people are involved. When you decide it’s right moment you start with launching your first attack from Vassal 1, when 2min (measuring with stopwatch*) pass you launch your attack from Vassal 2, after 3min from that moment you launch attack from Vassal 3 and finally 5min after Vassal 3 attack is launched you launch your raze/capture attack. This needs from you to have captain time shortest like in table above. In case where target is few ranks lower than you are you will lost two attacks and some good amount of honour, on the other side if you are launching captain last you’ll just lose two attacks. There is simple explanation for that if you start with launching where captain attack is first no one guarantees you that player will not come after your second launched attack online and interdict himself. I prefer attacks where captain attack is launched last. #STRONGHOLD KINGDOMS VILLAGE SIZE OFFLINE#*A – archers P – pikemens C – catapults Cap – Captain As you can see in table you have all data you need to launch your timed attack, your total launch time is 10min so you need guy to be offline and un-interdicted for 11min. – Collecting times and making timed attack Below you can see example times for one (3+1) attack: Launching spot ![]() When you have your times and you estimated you’ll need 3 attacks + raze (3+1) you are looking for best launching spots and if you haven’t filled them with troops you are doing so.ģ. After estimating how much attacks you’ll need you start with collecting times needed for your troops to reach target from villages, parishes, vassal armies (see chapter - 2). With some experience you’ll be able to estimate better, but for first few tries I would recommend you to send more you think is needed because it can’t be too much just too small. This data will help you to estimate number of attacks needed to have successful raze/capture. It is crucial to think about every possible detail you could find in specific situation like which rank is the player, has he possible assistance of any kind close to his village, is there possibility that guy uses cards, how often do you see him online, get his scout report etc. To make a successful attack you should make a good plan. – Planning of attacks This subject should take most of your time. ![]() Those “skilled” players who think this is a piece crap (I’m sure there will be some of them) I dedicate them first 33 seconds of this song. It could be that I forgot something to mention but I gave my best not to. You could do it step by step not all at once read practice as you go through. I tried to make it simple and short as I could but still there is pretty much of material which should be absorbed. #STRONGHOLD KINGDOMS VILLAGE SIZE HOW TO#– Introduction Hello there player, this is tutorial about how to become skilled attacker in Stronghold kingdoms. ![]() ![]() I'd hope they have more important things to do then enforce laws essentially brought up by software and record companies, but you never know. Required Downloads: PCSX2 PS2 BIOS (Mirror). #Play ps2 emulator bios install#Of course just because your doing something illegal doesn't mean the FBI will break down your door one day and seize your computer. Emulator PlayStation 2 PC windows linux Download /downloads/ Download and extract the PS2 BIOS, via /bios/ps2 Install the PCSX2.exe file Click Next next Enter the PS2 BIOS in the PCSX2 installation file (C:\Users\Computer\AppData\Local\Temp\RarEX01.610\PCSX2 1.6. PCSX2 supports controllers and ScpToolkit can be used to make Dualshock controllers compatible with Windows 7 / 10. I want to know who started that so I can slap them.Īnd If we extend the Fair-use clause toe Playstation BIOS files, that would mean it would only be legal if the BIOS file was actually generated from the Playstation that the person owned. 's PS2 BIOS download page (scph10000.zip). Like that old BS about ROM files being legal as long as you don't keep them on your computer for more than 24 hours. Since downloaded ROM files would not have been copied from your own cartridge, they thus are not enbodied in the Fair-use clause and are thus illegal- of course that doesn't stop you, but I'm getting tired of people essentially making up rules about what is and isn't illegal. PSP on PS2, though they have the same amount of power, doesnt mean they have the same coding put into their games. PSP is way to complicated right now to even run perfectly on high end computers. More than 1500 games are compatible with this PlayStation 2 emulator and more games are being made compatible every week. To answer your question, no, there are no emulators for the PSP and there isnt a way to play it on the PS2. No BIOS upload is required for running the emulator. ![]() #Play ps2 emulator bios how to#But for this tutorial I will show you how to use emulators for PS1, PS2 and PSP. Step 2: Drag the SCPH1001. Usually, you can do that by double-clicking the zip file. ![]() In short it allows you to play game of different gamming consoles on your PC. Step 1: Extract the BIOS file from the downloaded zip file. An emulator is a software that allows you to run PS1, PS2, PS3, PS3, PSP,Wii ,Xbox ,Game Cube ,Gameboy games on your PC. Actually, it works with Windows, Linux, macOS, iOS, and Android systems. For people who does not know what is an emulator. ![]() I am not sure if this applies to Playstation BIOS files, but I know with NES,SNES, and N64 cartridges, the "Fair use" clause essentially limits you to keep "backups" of the ROM, which must be generated from your cartridge. Play is a PS2 emulator for PC available in both 32-bit and 64-bit versions. It's actually a common misconception that you can legally download copyrighted material if you actually own the item that uses it. ![]() ![]() ![]() et al., “PTEN: a tumour suppressor that functions as a phospholipid phosphatase.”, Trends Cell Biol., 9(4):125-128 (USA, Apr. et al., “Targeting Akt in cancer therapy.”, Anticancer Drugs, 18(8):861-874 (USA, Sep. et al., “PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer.”, Science, 275(5308):1943-1947 (USA, Mar. USA, 95:15406-15411 (National Academy of Sciences, USA, Dec. et al., “PTEN/MMAC1/TEP1 suppresses the tumorigenicity and induces G1 cell cycle arrest in human glioblastoma cells”, Proc. A., “Membrane recognition by phospholipid-binding domains.”, Nat. et al., “Specificity and Promiscuity in Phosphoinositide Binding by Pleckstrin Homology Domains”, Journal of Biological Chemistry, 273(46):30497-30508 (USA, Nov. et al., “3-(Hydroxymethyl)-bearing phosphatidylinositol ether lipid analogues and carbonate surrogates block PI3-K, Akt, and cancer cell growth.”, J. et al., “Vav proteins, masters of the world of cytoskeleton organization.”, Cell Signal, 16(1):1-11 (Israel, Jan. G., “AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy.”, Nat. et al., “Role of substrates and products of PI 3-kinase in regulating activation of Rac-related guanosine triphosphatases by Vav.”, Science, 279(5350):558-560 (USA, Jan. et al., “PTEN can inhibit in vitro organotypic and in vivo orthotopic invasion of human bladder cancer cells even in the absence of its lipid phosphatase activity”, Oncogene, 23:6788-6797 (Nature Publishing Group, USA, 2004). et al., “PEG-PE/phosphatidylcholine mixed immunomicelles specifically deliver encapsulated taxol to tumor cells of different origin and promote their efficient killing.”, J. et al., “Roles of Rho-family GTPases in cell polarization and directional migration.”, Curr. et al., “Phosphoinositide kinases.”, Annu. et al., “TRAIL-R deficiency in mice promotes susceptibility to chronic inflammation and tumorigenesis”, Journ. et al., “Rho GTPases in cell biology.”, Nature, 420(6916):629-635 (United Kingdom, Dec. et al., “Akt Stimulates Aerobic Glycosis in Cancer Cells”, Cancer Research, 64:3892-3899 (USA, Jun. et al., “Cellular survival: a play in three Akts”, Genes & Development, 13:2905-2927 (USA, 1999).Įlstrom, R. et al., “Akt Phosphorylation of BAD Couples Survival Signals to the Cell-Intrinsic Death Machinery”, Cell, 91:231-241 (USA, Oct. P., “PIP2 and PIP3: Complex Roles at the Cell Surface”, Cell,100:603-606 (USA, Mar. et al., “Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B.”, Nature, 378(6559): 785-789 (United Kingdom, Dec. et al., “Preferential Inhibition of Akt and Killing of Akt-Dependent Cancer Cells by Rationally Designed Phosphatidylinositol Ether Lipid Analogues”, Cancer Research, 64:2782-2792 (USA, Apr. et al., “Regulation of cell death protease caspase-9 by phosphorylation.”, Science, 282(5392):1318-1321 (USA, Nov. J., et al., “Integrins in angiogenesis and lymphangiogenesis”, Nat. Interaction with inositol phosphates.”, J. et al., “Solution structure and backbone dynamics of the pleckstrin homology domain of the human protein kinase B (PKB/Akt). et al., “Pulmonary delivery of peptide drugs: effect of particle size on bioavailability of leuprolide acetate in healthy male volunteers.”, Pharm. et al., “The complexity of TNF-related apoptosis-inducing ligand.”, Ann. ac.uk/MSDS structure activity relationship Jaworska, 1-8, 2004.Ībe, K. Cecil Text book of Medicine, 20th Edition vol. ![]() ![]() Since you have already explored the entire map, everything will have been rendered already, there is no risk that this change will reset any of the important coordinates.Ĭlick here to view the Minecraft seed list for all versions of the game. Id love to see the 1:1 or 1:1500 Planet Earth maps seed added to the seed. In SMP, you can use the same command if you have sufficient rights. Alternatively, you can use the /seed command ingame. If you're playing SSP, the app is able to fetch the seed from your savegame. From here, go into the settings for the level and change the world size to large. There should be a seed in Minecraft example: 752638032784 (Plant Earth) that. Seed For technical reasons, you need to know the seed of your world to use Seed Map, unless, of course, you want to find a seed for a new world. Once you are happy you have explored all of the important areas of the map, you can exit back to the main menu. Once the world has been rendered, it is saved to your game save and no updates to the game will ever change it. Once you are in the world, you must run around the map and make sure all of the world has been rendered. To use it with a large world, you must first follow the instructions on the seed page and load the world up in classic mode. Chances are if you are going through the list of best seeds for Minecraft console edition, you will come across a classic one. This is done to make sure that the map will work on as many systems as possible and because landmarks spawn more frequently in these seeds as they have more packed into a smaller area. Using Large World Size For Classic World SeedsĪ large amount of well documented seeds for Minecraft Console Edition are built with a classic world size. You will keep all of the same map features and all of the locations at the coordinates specified in the seed post. Changing the size will not alter the world that has already loaded. The key piece of information here is that you can easily change the map size after you have created a world. The easier way to find the best seeds that include rare and amazing biome systems Request any Minecraft seed and a map will magically appear Exclusive map. It is actually very easy to increase the size of classic world seeds and enjoy any seed using the largest possible world map. If the classic size map is an issue for you and you are playing on the PS4, Xbox One or any system that is using a game version above the Legacy Console version, you can actually bypass this limit. If that seed was used on the PlayStation 3 or any of the legacy console versions of the game then you will have no choice but to set the world size to classic if you want to use it on one of the newer systems. The app also requires an Internet connection.If you are playing the console edition on the newer systems you may come across a well documented Minecraft seed that you want to use. This app contains advertisements (which can be removed for a small fee). It is not endorsed or sponsored by Mojang AB. We have many new exciting features in development! Stay tuned and we hope you enjoy using this app. This is the first version of the ultimate seeds map app. Save your favorite maps for easy access later.Points of interest - explore the maps to discover amazing points of interest, such as: biomes, villages, witches, etc.explore the world with our topographical maps! Request any Minecraft seed and a map will magically appear!.The easier way to find the best seeds that include rare and amazing biome systems!.Players may utilize a Seed Map to find and move. We don't just provide normal screenshots, we let you explore the world using our familiar map like system! You won't find an app with higher quality topographical Minecraft maps that ours. Find Minecraft Pocket Edition Seed Map: In Minecraft, a seed is the starting point for creating a world. #SEED MAP MINECRAFT CODE#Seeds Map for Minecraft is the quickest and FREE* way to discover amazing Minecraft™ seeds. Tuesday, AugMinecraft Seed Map Viewer Minecraft seeds are the code numbers used to identify each new spawn of a Minecraft world. ![]() ![]() Then you will get an injection (shot) of local anesthesia (numbing medication) into the area so that you won’t feel any pain. Your healthcare provider will clean the area around your lower back with an disinfecting solution. It’s very important to stay still during the procedure. What to expectįor your procedure, you will either be lying on your side and hugging your legs or sitting up and leaning over a table so that your back is curved in a “C” shape. There are others, so be sure your doctor knows all the medications you’re taking.īack to top The Day of Your Procedure Where to goĬheck your appointment reminder for information on where to go. Some examples of blood thinners are heparin, warfarin (Coumadin ®), clopidogrel (Plavix ®), enoxaparin (Lovenox ®), and tinzaparin (Innohep ®). Sensitive or allergic to any adhesives such as tape.Allergic to iodine (an ingredient in Betadine ®, an antiseptic).Cancer-fighting medications (such as chemotherapy).Anesthesia (medications that block pain).Your healthcare provider may also do a lumbar puncture to put certain types of medication directly into the area surrounding your spinal cord. Cancer that has spread to the tissues around your brain or spinal cord.Bleeding in your brain or spinal canal.An infection, such as meningitis (an infection where the tissues around your brain and spinal cord swell).Your healthcare provider may need to take a sample of your CSF to check for problems such as: A lumbar puncture can also be called a “spinal tap” or an “LP.” CSF is the fluid that surrounds your brain and spinal cord. This information will explain your lumbar puncture procedure at Memorial Sloan Kettering (MSK).Ī lumbar puncture is a procedure that’s done to get a sample of cerebrospinal fluid (CSF). ![]() |
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